CURRICULUM VITAE


Family name:                               Arseniev
First name:                                Alexander
Second name:                               Sergeevich
Date of birth:                             January 1, 1952
Nationality:                               Russian

Address, telephone, E-mail and telex:

Professor Alexander S. Arseniev
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry
Russian Academy of Sciences
Ul. Miklukho-Maklaya, 16/10
117871 GSP-7 Moscow V-437
Russian Federation

Phone: (007)(095)330-5929;
FAX: (007)(095)335-50-33 or 335-71-03
E-mail: aars@nmr.ru
Telex: 411982 trem su

Education:

1973 - M.Sc. in Chemistry (Belorussian State University, Faculty of Chemistry, Minsk, Belarus), Diploma thesis "EPR study of metalloporphirine cation-radicals"

1979 - PhD in Chemistry (Shemyakin Institute of Bioorganic Chemistry, Moscow, Russian Federation), PhD thesis "NMR study of spatial structure of neurotoxin II Naja naja oxiana"

1988 - DR.Sc. in Chemistry (Shemyakin Institute of BioorganicChemistry, Moscow, Russian federation), Doctor of Sciences thesis "2D NMR spectroscopy and computer modeling as a tool of protein spatial structure analysis"

Professional experience:

1971-1973: student of Chemistry Faculty of Belorussian State University

1973-1976: post graduated student, Shemyakin Institute of Bioorganic Chemistry (conformational NMR analysis of polypeptide toxins: neurotoxins, cardiotoxins, apamin )

1976-1980: Junior scientist, Shemyakin Institute of Bioorganic Chemistry (conformational NMR analysis of polypeptide toxins and ESR study of mechanisms of interaction of neurotoxin II N. n. oxiana with acetylcholine receptor Torpedo marmorato)

1981: postdoctoral Fellow, Eidgenossische Technische Hochschule, Institute fur Molekularbiologie und Biophysik, Zurich, (Prof. K. Wuthrich) ( Assignment of the 1H NMR spectrum of the trypsin inhibitor E D. p. polylepis. 2D NMR spectroscopy).

1982 - 1986: Senior scientist, Shemyakin Institute of Bioorganic Chemistry (2D NMR analysis of spatial structure of insectotoxin I5A Buthus eupeus, neurotoxin I N. n. siamensis, gramicidin A (double helices, complex with caesium, trasnsmembrane ion channel)).

1986: visiting Fellow, Eidgenossische Technische Hochschule, Institute fur Molekularbiologie und Biophysik, Zurich, (Prof. K. Wuthrich) (2D NMR study of spatial structure of metallothionein)

1986-1990: Researcher, Shemyakin Institute of Bioorganic Chemistry (NMR analysis of spatial structure of bacteriorhodopsin H. halobium and ion-channel state of gramicidin A and its analogs)

1991 - 1993: Vice-president of the Shemyakin Institute of Bioorganic Chemistry

1994 - Visyting Professor CABM, Rutgers, the State University of New Jersey.

1991 - present time: Head of NMR laboratory of Shemyakin Institute of Bioorganic Chemistry. Spatial structure of membrane and water soluble peptides and proteins, peptide design, peptide-protein recognition. Used techniques: NMR, ESR, optical spectroscopy, molecular modeling, molecular dynamics. Special interest in protein structure and dynamics by NMR relaxation and computer modelling.

1991 - present time: Head of Department of Physical Chemical Biology and Biotechnology of the Moscow Physical Technical Institute. Lecture course on NMR in bioorganic chemistry.

Main research interests:

NMR spectroscopy, Spatial structure and dynamics of water soluble and membrane peptides and proteins

Studied objects: polypeptide toxins (from bee, ant, scorpion and snake venoms), nicotinic acetylcholine receptor, phospholamban, bacteriorhodopsin, gramicidin A, trypsin inhibitor, metallothionein, ribosomal protein L7/L12.

Publications: over 200 in Russian and international journals and books.

Awards, professional societies:

1993 - Ovchinnikov prize in Physical Chemical Biology (Russian academy of Sciences) for NMR research of membrane peptides and proteins.

1996 - Award of Life Sciences and Biotechnology Council (Russian Academy of Sciences and Ministery of Sciences and technical politics) for Spatial structure and dynamics of membrane and membrane active proteins.

Member of Russian academy of Sciences Council for Bioorganic Chemistry (1991 - present time)

Member of Russian academy of Sciences Council for Biophysics (1992 - present time)

Member of Council of Russian State Program on Protein Engineering (1989 - present time),

Member of Scientific Council at the Shemyakin Institute of Bioorganic Chemistry (1989 - present time).

Member of the Editorial Board of Journal of Biomolecular NMR (1991 - present time)

Member of the Editorial Board of Journal of Bioorganicheskaya Khimiya (Russia) (1991 - present time).

List of selected publications:

1. Proton NMR Study of the conformation of neurotoxin II from Central Asian Cobra (Naja naja oxiana) venom. ( A.S. Arseniev, T.A. Balashova, V.F. Bystrov, Yu. N. Utkin, V.I.Tsetlin, V.T. Ivanov, Yu.A. Ovchinnikov) Eur. J. Biochem. (1976), v.71, p.595.
2. NMR Spectroscopy of Large Peptides and Small Proteins (V.F. Bystrov, A.S. Arseniev and Yu.D. Gavrilov) J. Magn. Reson. (1978), v.30, p.151.
3. EPR and Fluorescence Study of Interaction of Naja naja oxiana neurotoxin II and its derivatives with acetylcholine receptor protein from Torpedo marmorato. (V.I. Tsetlin, E. Karlsson, A.S. Arseniev, Yu. N. Utkin, A.M. Surin, V.S. Pashkov, K.A. Pluzhnikov, V.F. Bystrov, V.T. Ivanov, Yu.A. Ovchinnikov) FEBS Letters (1979) v.106, p.47.
4. 19F NMR Determination of intramolecular Distances in Spin and fluorine labeled proteins. Neurotoxin II Naja naja oxiana. (A.S. Arseniev, Yu. N. Utkin, V.S. Pashkov, V.I. Tsetlin, V.T. Ivanov, V.F. Bystrov, Yu. A. Ovchinnikov) FEBS Letters (1981) v.136, p.269.
5. Assignment of the 1H Nuclear Magnetic Resonance Spectrum of the Trypsin Inhibitor E from Dendroaspis polylepis polylepis. Two- dimensional NMR at 500 MHz. ( A.S. Arseniev, G. Wieder, F.J. Joubert, K. Wuthrich) J. Mol. Biol. (1982) v.159, p.323.
6. NMR Solution Spatial Structure of "short" scorpion Insectotoxin I5A (A.S. Arseniev, V.I. Kondakov, V.N. Maiorov, V.F. Bystrov) FEBS Letters (1984) v.165, p.57.
7. Combined COSY-NOESY Experiment (A.Z. Gurevich, I.L. Barsukov, A.S. Arseniev, V.F. Bystrov) J. Magn. Reson. (1984) v.58, p.471.
8. NMR Solution Conformation of Gramicidin A Double Helix (A.S. Arseniev, V.F. Bystrov, V.T. Ivanov, Yu.A. Ovchinnikov) FEBS Letters (1984) v. 165, p.51.
9. NMR Solution Structure of Gramicidin A Complex with Caesium Cation (A.S. Arseniev, I.L. Barsukov, V.F. Bystrov) FEBS Letters (1985) v.186, p.168.
10. T1 Relaxation Measurement by Two-Dimensional NMR Spectroscopy (A.S. Arseniev, A.G. Sobol, V.F. Bystrov) J. Magn. Reson. (1986) v.70, p.427.
11. 1H-NMR study of gramicidin A transmembrane ion channel. Head-to-head right-handed, single-stranded helices. (A.S. Arseniev, I.L. Barsukov, A.L. Lomize, V.F. Bystrov, Yu. A. Ovchinnikov) FEBS Letters (1985) v.186, p.168.
12. 19F NMR study of 5-fluorotryptophan-labeled bacteriorhodopsin ( A.S. Arseniev, A.B. Kuryatov, V. I. Tsetlin, V.F. Bystrov, V.T. Ivanov, Yu.A. Ovchinnikov) FEBS Letters (1987) v.213, p.283.
13. 2D NMR of Single and Double Stranded Helices of Gramicidin A in Micelles and Solutions (V.F. Bystrov, A.S. Arseniev, I.L. Barsukov, A.L. Lomize): Bull. Magn. Reson. (1987) v.8, p.84.
14. Two-dimensional 1H NMR Study of (34-65)bacterioopsin polypeptide conformation. (A.S. Arseniev, I.V. Maslennikov, V.F. Bystrov, A.T. Kozhich, V.T. Ivanov, Yu. A. Ovchinnikov) FEBS Letters (1988) v.231, p.81.
15. Three dimensional structure of rabbit liver [Cd7]metallo- thionein 2a in aqueous solution determined by NMR. (A. Arseniev, P. Shulze, E. Worgotter, W. Braun, G. Wagner, M. Vasak, J.H.R. Kagi, K. Wuthrich) J. Mol. Biol. (1988) v.201, p.637.
16. Spatial Structures of Gramicidin A in Organic Solvents. H-NMR Analysis of Four Species in Ethanol (I.L. Barsukov, A.S. Arseniev, V.F. Bystrov): Bioorgan. Khim.(USSR) (1987) v.13, p.1501.
17.Diversity of the Gramicidin A Spatial Structure: Two-Dimensional 1H NMR Study in Solution (V.F. Bystrov, A.S. Arseniev) Tetrahedron (1988) v.44, p.925.
18. Isolation and structure analysis of components from venom of the spider Argiopa lobata (Grishin E.V., Volkova T.M., Arseniev A.S.) Toxicon (1989), v.27, p.541.
19. Sequence specific 1H NMR assignment and conformation of proteolytic fragment 163-231 of bacteriorhodopsin. (I.L. Barsukov, G.V. Abdulaeva, A.S. Arseniev, V.F. Bystrov. ) Eur. J. Biochem. (1990) v.192, p.321.
20. The divalent cation-binding sites of gramicidin A transmembrane ion channel. (Golovanov A.P., Arseniev A.S., Barsukov L.I., Sukhanov S.V., Bystrov V.F.) Biopolymers (1991) v.31, p.425.
21. Two-dimensional NMR study of the conformation of (34-65) bacteriorhodopsin polypeptide in SDS micelles. ( Pervushin K.V., Arseniev A.S., Kozhich A.T., Ivanov V.T.) J. Biomol. NMR (1991), v.1, p.313.
22. Sequence-specific resonance assignment and secondary structure of (1-71)bacterioopsin. (Sobol A.G., Arseniev A.S., Abdulaeva G.V., Bystrov V.F.) J. Biomol. NMR (1992) v.2, p.161.
23. Three-dimensional structure of proteolytic fragment 163-231 of bacterioopsin determined from nuclear magnetic resonance data in solution. (Barsukov I.L., Nolde D.E., Lomize A.L., Arseniev A.S.) Eur. J. Biochem. (1992) v.206, p.665.
24. 1H-15N NMR study of bacteriorhodopsin Halobacterium halobium. Conformational dynamics of the four helical bundle.( Orekhov V.Yu., Abdulaeva G.V., Musina L.Yu., Arseniev A.S.) Eur. J. Biochem. (1992) v.210, p.223.
25. Spatial structure of (34-65) bacterioopsin polypeptide in SDS micelles determined from nuclear magnetic resonance date. (Lomize A.L., Pervushin K.V., Arseniev A.S.) J. Biomol. NMR (1992) v.2, p.361.
26. Three-dimensional structure of (1-36) bacterioopsin in methanol-chloroform mixture and SDS micelles determined by 2D H-NMR spectroscopy. (Pervushin K.V., Arseniev A.S.) FEBS Letters (1992) v.308, p.190.
27. Two-dimensional H-NMR study of neurotoxin II Naja naja oxiana spatial structure. (Golovanov A.P., Lomize A.L., Arseniev A.S., Utkin Yu.N., Tsetlin V.I.) Eur. J. Biochem. (1993) v.213, p.1213. 28. 3D structure of (1-71)bacterioopsin solubilized in organic mixture and SDS micelles determined by H- N NMR. (Pervushin K.V., Orekhov V.Yu., Popov A.I., Musina L.Yu and Arseniev A.S.) Eur. J. Biochem. (1994) v.219, p.571.
29. Backbone dynamics of (1-71)bacterioopsin studied by two dimensional H- N NMR spectroscopy. (Orekhov V.Yu., Pervushin K.V., Arseniev A.S.) Eur. J. Biochem. (1994) v. 219, p.887.
30. Structural and functional studies of toxin principle of Ectatomma tuberculatum ant venom& (Pluzhnikov K.A. Nolde D.E., Tertyshnikova S.M. Sukhanov S.V., Sobol A.G., Torgov M.Yu., Fislippov A.K. Arseniev A.S., Grishin E.V.) Bioorgan. Khim. (Russia) (1994) v. 20 p. 857
31. Toxic principle of selva ant venom is a pore forming protein-transformer. (Arseniev A.S., Pluzhnikov K.A., Nolde D.E., Sobol A.G., Torgov M.Yu., Sukhanov S.V., Grishin E.V.) FEBS Letters (1994) v. 347, p. 112.
32. Three-dimensional structure of ectatomin from Ectatomma tuberculatum ant venom. (Nolde D.E., Sobol A.G., Pluzhnikov K.A., Grishin E.V., Arseniev A.S.) J. Biomol. NMR (1995) v. 5, p. 1. 33. An estimate of spin diffusion in a spin subset: Application to iterative distance calculation from 3D N NOESY-HMQC. (Malliavin T.E., Delsuc M.A., Orekhov V.Yu., Arseniev A.S.) J. Biomol. NMR (1995) v.5, p. 193.
34. Manifestation of intramolecular motions on pico- and nanosecond time scales in H- N NMR relaxation: Analysis of dynamic models of one- and two-helical subunits of bacterioopsin. (Pervushin K.V., Orekhov V.Yu., Korzhnev D.M., Arseniev A.S.) J. Biomol. NMR (1995) v. 5, p. 383.
35. Detailed assessment of spatial hydrophobic and electrostatic properties of 2D NMR-derived models of neurotoxin II. (Efremov R.G., Golovanov A.P., Vergoten G., Alix A.J.P., Tsetlin V.I., Arseniev A.S.) J. Biomolec. Struct. & Dynamics (1995) v. 12, p. 971.
36. Advances of the NMR Spectroscopy in the spatial structure determination of membrane associated peptides and proteins. (Pervushin K.V., Arseniev A.S.) Bioorgan. Khim. (Russia) (1995) v. 21,p. 83.
37. Refinement of the spatial structure of neurotoxin II from Naja naja oxiana venom& (Golovanov A.P., Arseniev A.S.) Bioorgan. Khim. (Russia) (1995) v. 21, p. 571.
38. Studies of the interaction of Na ,K -ATPase by Na NMR Spectroscopy. (Pashkov V.S., Balashova T.A., Dzhandzhugazyan K.N., Arseniev A.S.) Membr. and Cell Biol. (1995) v.8, p. 523.
39. Backbone dynamics of (1-71)- and (1-36)bacterioopsin studied by two-dimensional H- N NMR spectroscopy. (Orekhov V.Yu., Pervushin K.V., Korzhnev D.M., Arseniev A.S.) J. Biomol. NMR, (1995) v. 6, p. 113.
40. Spatial structure of transmembrane segments C, E and G of bacterioopsin by two-dimensional H NMR spectroscopy. (Maslennikov I.V. Bocharov E.V. Arseniev A.S.) Bioorgan. Khim. (Russia) (1995) v. 21, p. 659.
41. Amino acid residue: is it structural or functional? (Golovanov A.P., Efremov R.G., Jaravine V.A., Vergoten G., Arseniev A.S.) FEBS Letters (1995) v. 375, p. 162.
42. Processing of heteronuclear NMR relaxation data with the new software DASHA. (Orekhov V.Yu., Nolde D.E., Golovanov A.P., Korzhnev D.M., Arseniev A.S.) Appl. Magn. Reson. (1995) v. 9, p. 581.
43. The secondary structure of phospholamban: a two-dimensional NMR study. (Maslennikov I.V., Sobol A.G., Anagli J., James P., Vorherr T., Arseniev A.S., Carafoli E.) Biochem. Biophys. Res. Commun. (1995) v. 217, p. 1200.
44. Topology of the structure elements of ribosomal protein L7/L12 from E. coli in solution. (E.V. Bocharov, A.T. Gudkov, A.S. Arseniev) FEBS Letters (1996) v. 379, p. 291.
45. Conformation of surface exposed N-terminus part of bacteriorhodopsin studied by transferred NOE technique. (V.S. Pashkov, T.A. Balashova, L.V. Zhemaeva, N.N. Sikilinda, M.A. Kutuzov, N.G. Abdulaev, A.S. Arseniev) FEBS Letters (1996) v. 381, p. 119.